Acute toxicity test of water and ethanol extract from african leaf (Gym-nanthemum amygdalina Del.) on zebra fish embrio (Danio rerio)

Bitterleaf or African Leaf is a herbal plant empirically used to relieve fever and kidney disease. Pharmacological research reports that the leaves have antibacterial, anti-inflammatory, antidiabetic properties, toxicity test was also conducted ensure their safety. Therefore, this experiment aimed obtain LC50 of (Gymnanthemum amygdalina Del.) ethanol water extract with ZFET (Zebra Fish Embryo Acute Toxicity) method observe effect zebrafish (Danio rario) embryo's morphology after induction extract. The refers OECD No.236 2013 as guideline, it shown Leaf's values were obtained at 6.3629 ppm. Furthermore, value 25.0520 ppm, categorized in toxic category. leaf has harmful on embryos, resulting malformations tail, notochord, pericardium, yolk sac.